ATP2 is a prediction tool trained with sequences of plant proteins dually (ambiguously) targeted to plastids and mitochondria, to predict such localization in plant proteins.
GTP_Pp is a prediction tool trained with sequences from Physcomitrella patens to predict subcellular targeting of P. patens/plant proteins.
GTP_Ref is a prediction tool trained mainly with seed plant sequences from the TargetP training set to predict subcellular targeting of plant proteins.
Fuss J., Liegmann O., Krause K., Rensing S.A. (2013) Green Targeting Predictor and Ambiguous Targeting Predictor 2: the pitfalls of plant protein targeting prediction and of transient protein expression in heterologous systems. New Phytol 200:1022
Please insert protein sequences in FASTA format. You can enter them directly into the input field or upload them as a file. The input sequences need to be at least 71 amino acids long. All shorter sequences will be disregarded. You can predict at most 50 sequences at a time. If you like to predict a large number of sequences, please contact us at email@example.com.
For proteins with low GTP values for MIT or CHL and/or low confidence scores we suggest to check by ATP2 whether the protein might be ambiguously targeted to these two compartments.