GeBP

Description

Curaba et al. (2003): Trichomes of Arabidopsis are single-celled epidermal hair that are a useful model for studying plant cell fate determination. Trichome initiation requires the activity of the GLABROUS1 (GL1) gene whose expression in epidermal and trichome cells is dependent on the presence of a 3'-cis-regulatory element. Using a one-hybrid screen, we have isolated a cDNA, which encodes for a protein, GL1 enhancer binding protein (GeBP), that binds this regulatory element in yeast and in vitro. GeBP and its three homologues in Arabidopsis share two regions: a central region with no known motifs and a C-terminal region with a putative leucine-zipper motif. We show that both regions are necessary for trans-activation in yeast. A translational fusion with the Yellow Fluorescent Protein (YFP) indicates that GeBP is a nuclear protein whose localization is restricted to, on average, 3-5 subnuclear foci that might correspond to nucleoli. Transcriptional fusion with the GUS reporter indicates that GeBP is mainly expressed in vegetative meristematic tissues and in very young leaf primordia. We looked at GeBP expression in plants mutated in or misexpressing KNAT1, a KNOX gene, expressed in the shoot apical meristem and downregulated in leaf founder cells, and found that GeBP transcript level is regulated by KNAT1 suggesting that KNAT1 is a transcriptional activator of GeBP. This regulation suggests that GeBP is acting as a repressor of leaf cell fate.

References:


1) Chevalier, F; Perazza, D; Laporte, F; Le Hénanff, G; Hornitschek, P; Bonneville, JM; Herzog, M; Vachon, G. 2008. GeBP and GeBP-like proteins are noncanonical leucine-zipper transcription factors that regulate cytokinin response in Arabidopsis. Plant Physiol. 146(3):1142-54 PubMed
2) Curaba, J; Herzog, M; Vachon, G. 2003. GeBP, the first member of a new gene family in Arabidopsis, encodes a nuclear protein with DNA-binding activity and is regulated by KNAT1. Plant J. 33(2):305-17 PubMed


Summary

Name: GeBP
Class: TF
Number of species containing the TAP: 91
Number of available proteins: 927

The colour code corresponds to the rules for the domains:

should be contained
should not be contained

Domain rules:


(Domain names are clickable)


Phylogenetic tree for Archeaplastida:

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TAP distribution:

The following table shows the distribution of GeBP over all species included in TAPscan. The values for e.g. a specific kingdom are shown in the tree below if you expand the tree for that kingdom.

Minimum Maximum Average Median Standard deviation
0407.6467.69

List of species containing GeBP sorted by kingdomcladesupergrouporderfamily:

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expand kingdom Archaeplastida (940 proteins in 91 species)
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A list of species letter codes included in the protein names can be found here (opens in new tab).


Impressum