PLATZ

Description

Nagano et al. (2001): Complementary DNA encoding a DNA-binding protein, designated PLATZ1 (plant AT-rich sequence- and zinc-binding protein 1), was isolated from peas. The amino acid sequence of the protein is similar to those of other uncharacterized proteins predicted from the genome sequences of higher plants. However, no paralogous sequences have been found outside the plant kingdom. Multiple alignments among these paralogous proteins show that several cysteine and histidine residues are invariant, suggesting that these proteins are a novel class of zinc-dependent DNA-binding proteins with two distantly located regions, C-x(2)-H-x(11)-C-x(2)-C-x((4-5))-C-x(2)-C-x((3-7))-H-x(2)-H and C-x(2)-C-x((10-11))-C-x(3)-C. In an electrophoretic mobility shift assay, the zinc chelator 1,10-o-phenanthroline inhibited DNA binding, and two distant zinc-binding regions were required for DNA binding. A protein blot with (65)ZnCl(2) showed that both regions are required for zinc-binding activity. The PLATZ1 protein non-specifically binds to A/T-rich sequences, including the upstream region of the pea GTPase pra2 and plastocyanin petE genes. Expression of the PLATZ1 repressed those of the reporter constructs containing the coding sequence of luciferase gene driven by the cauliflower mosaic virus (CaMV) 35S90 promoter fused to the tandem repeat of the A/T-rich sequences. These results indicate that PLATZ1 is a novel class of plant-specific zinc-dependent DNA-binding protein responsible for A/T-rich sequence-mediated transcriptional repression.

References:


Nagano, Y; Furuhashi, H; Inaba, T; Sasaki, Y. 2001. A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. Nucleic Acids Res. 29(20):4097-105 PubMed


Summary

Name: PLATZ
Class: TF
Number of species containing the TAP: 115
Number of available proteins: 1337

The colour code corresponds to the rules for the domains:

should be contained
should not be contained

Domain rules:


(Domain names are clickable)


Phylogenetic tree for Archeaplastida:

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TAP distribution:

The following table shows the distribution of PLATZ over all species included in TAPscan. The values for e.g. a specific kingdom are shown in the tree below if you expand the tree for that kingdom.

Minimum Maximum Average Median Standard deviation
03610.98117.93

List of species containing PLATZ sorted by kingdomcladesupergrouporderfamily:

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expand kingdom Archaeplastida (1350 proteins in 115 species)
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A list of species letter codes included in the protein names can be found here (opens in new tab).


Impressum